Qualifications and Career
Degree programme | 2001-2006 | Biochemistry, Potsdam, Germany |
Doctorate | 2006–2010 | Prof. Dr. Albert J.R. Heck, ‘Analysing macromolecular structures with native mass spectrometry: Insights to virus structure and assembly’, Biomolecular Mass Spectrometry and Proteomics, Utrecht University, Utrecht, the Netherlands |
Stages of academic/ professional career | Since 04/2024 | Professor for Chemistry, Institute of Chemistry and Metabolomics, Universität zu Lübeck, Lübeck, and Group Leader ‘Dynamics of Viral Structures’ at UzL, Lübeck, DESY and CSSB, Hamburg, Germany |
2021-2024 | Associate Professor (W2) for Biochemistry, University of Siegen, Siegen, and Group Leader ‘Dynamics of Viral Structures’ at University of Siegen, Siegen, LIV, DESY and CSSB, Hamburg, Germany | |
2020-2021 | Associate member, CSSB, Hamburg, Germany | |
2014-2021 | Junior Group Leader ‘Dynamics of Viral Structures’, Leibniz Institute of Virology (LIV), Hamburg, Germany | |
2011-2022 | Guest Scientist (Postdoc, 2013–2014), Sample Environment, European XFEL GmbH, Schenefeld, Germany | |
2011-2013 | Postdoc, EMBO longterm fellowship, Molecular Biophysics, Uppsala University, Uppsala, Sweden |
Research Topics
- Norovirus capsid dynamics
- Coronaviral replication/transcription complexes
- Mass spectrometry based single molecule approaches
Activities in the Research System
Since 2024 | Director (jointly with U. Günther) of the Institute of Chemistry and Metabolomics, Universität zu Lübeck, Germany |
2024-2025 | Deputy Scientific Director, Centre for Structural Systems Biology (CSSB), Hamburg, Germany |
2024 | Chair of the 2024 Isolated Biomolecules and Biomolecular Interactions, Germany |
2023-2027 | Coordinator of the ‘The next generation SPIDoc’s Doctoral Network’ funded by the Marie Skłodowska Curie Action program HORIZON-MSCA-2022-DN under grant agreement no. 101120312 |
2023-2024 | Member of Council of Research Ethics of the University of Siegen, Germany |
2023 | Chair of the 2023 GRC Physical Virology, Italy |
2022-2027 | Member of Steering Committee, DFG Research Training Group (RTG) 2771 ‘Humans and Microbes: Reorganisation of Cell Compartments and Molecular Complexes during Infection’ |
2022-2024 | Member of Analytical Chemistry Early Career Board, American Chemical Society |
2021-2023 | Selected international member of Virology Division, Microbiology Society, United Kingdom |
Since 2020 | Member of Internal Advisory Board of the CryoEM multiuser facility at CSSB, Hamburg, Germany |
2019-2023 | Member of Board of Directors, Leibniz ScienceCampus ‘InterACt – Integrative analysis of pathogen-induced compartments’, Hamburg, Germany |
2019-2021 | Deputy chair of Scientific Council of the Leibniz Institute of Virology (LIV), Hamburg, Germany |
2018-2023 | Coordinator of the project ‘MS SPIDOC’ funded by the European Union's Horizon 2020 research and innovation programme under grant agreement no. 801406 |
Since 2017 | Jury member for the ‘Wolfgang-Paul-Studienpreis’ of the German Mass Spectrometry Society (Deutsche Gesellschaft für Massenspektrometrie, DGMS) |
Since 2015 | External expert for the European Commission in various funding schemes and for other funding agencies in Europe |
2015 | Organiser of the ‘CSSB Mini Symposium: Native Mass Spectrometry’, Hamburg, Germany |
Supervision of Researchers in Early Career Phases
Since 2014 | Postdoctoral scientists (14) / PhD students (17, of which 8 defended) / Master students (10) / several Bachelor students at LIV, University of Siegen and Universität zu Lübeck, Germany. One former PhD student and two postdoctoral scientists are now transitioning to independent PIs |
Since 2014 | Active mentor not only in own lab but also in many thesis advisory committees and in the context of formal mentorship programmes |
Scientific Results
Category A
(# indicates corresponding authors)
Sänger L, Williams HM, Yu D, Vogel D, Kosinski J, Rosenthal M#, Uetrecht C# (2023) RNA to Rule Them All: Critical Steps in Lassa Virus Ribonucleoparticle Assembly and Recruitment. J Am Chem Soc 145(51):27958-27974 doi: 10.1021/jacs.3c07325
Kopicki JD, Saikia A, Niebling S, Günther C, Anjanappa R, Garcia-Alai M, Springer S#, Uetrecht C# (2022) Opening opportunities for Kd determination and screening of MHC peptide complexes. Commun Biol 5(1):488 doi: 10.1038/s42003-022-03366-0
Yan H, Lockhauserbäumer J, Szekeres GP, Mallagaray A, Creutznacher R, Taube S, Peters T, Pagel K, Uetrecht C#(2021) Protein Secondary Structure Affects Glycan Clustering in Native Mass Spectrometry. Life 11(6):554 doi: 10.3390/life11060554
Dülfer J, Yan H, Brodmerkel MN, Creutznacher R, Mallagaray A, Peters T, Caleman C, Marklund EG, Uetrecht C#(2021) Glycan-Induced Protein Dynamics in Human Norovirus P Dimers Depend on Virus Strain and Deamidation Status. Molecules 26(8):2125 doi: 10.3390/molecules26082125
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C# (2021) Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes. Sci Adv 7(10):eabf1004 doi: 10.1126/sciadv.abf1004
Pogan R, Weiss VU, Bond K, Dülfer J, Krisp C, Lyktey N, Müller-Guhl J, Zoratto S, Allmaier G, Jarrold MF, Muñoz-Fontela C, Schlüter H, Uetrecht C# (2020) N-terminal VP1 Truncations Favor T = 1 Norovirus-Like Particles. Vaccines 9(1):8 doi: 10.3390/vaccines9010008
Krichel B, Falke S, Hilgenfeld R, Redecke L, Uetrecht C# (2020) Processing of the SARS-CoV pp1a/ab nsp7-10 region. Biochem J. 477(5):1009-1019 doi: 10.1042/BCJ20200029
Uetrecht C#, Lorenzen K, Kitel M, Heidemann J, Robinson Spencer JH, Schlüter H, Schulz J (2019) Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging. J Synchrotron Radiat. 26(Pt 3):653-659 doi: 10.1107/S1600577519002686
Uetrecht C, Barbu IM, Shoemaker GK, van Duijn E, Heck AJR (2011) Interrogating viral capsid assembly with ion mobility-mass spectrometry. Nat Chem. 3(2):126-132 doi: 10.1038/nchem.947
Uetrecht C, Watts NR, Stahl SJ, Wingfield PT, Steven AC, Heck AJR (2010) Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent. Phys Chem Chem Phys 12(41):13368-13371 doi:10.1039/c0cp00692k
Category B
(# indicates corresponding authors)
Editor Viruses Special Issue in 2023: Physical Virology
Norovirus Superstar (in English or German). Mischitz V. Editors: Cross A, Wissel S, Uetrecht C, Lorenzen K. Comic book for the EU project MS SPIDOC. doi: 10.22003/XFEL.EU-COMIC-2021-001-EN / doi: 10.22003/XFEL.EU-COMIC-2021-001-DE
Feng Y, van Bodegraven D, Kádek A, Munguira ILB, Soria-Martinez L, Nentwich S, Kavan D, Uetrecht C#, Schelhaas M#, Roos WH# (2024) Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility. bioRxiv 2024.02.01.577804 doi: 10.1101/2024.02.01.577804
Schamoni-Kast K, Krichel B, Damjanović T, Kierspel T, Toker S, Uetrecht C# (2024) The kinetics of SARS-CoV-2 nsp7-11 polyprotein processing and impact on complexation with nsp16. bioRxiv 2024.01.06.574466 doi: 10.1101/2024.01.06.574466
Academic Distinctions
2022 | Mattauch-Herzog Award of the DGMS |
2017 | ERC Starting Grant ‘SPOCkS MS’ (Sampling Protein complex Conformational Space with native top down Mass Spectrometry) |
2014 | Leibniz Competition Grant ‘Promoting women in academic leadership positions’ of the Leibniz Association |
2011 | H.G.K. Westenbrink Prize of the Netherlands Society for Biochemistry and Molecular Biology (NVBMB) for the best thesis of the year 2010 |
2010 | Ph.D. with Cum Laude (highest degree in the Netherlands awarded to < 5% of doctorates) |
2010 | EMBO Long-term Fellowship ALTF 1531-2010 |
Full list of publications
See also: ResearcherID D-1883-2010 or ORCID 0000-0002-1991-7922
Peer-review original articles
- Collision induced unfolding and molecular dynamics simulations of norovirus capsid dimers reveal strain-specific stability profiles. Brodmerkel M, Thiede L, de Santis E, Uetrecht C, Caleman C, Marklund EM. (2024) Phys Chem Chem Phys, DOI: 10.1039/D3CP06344E
- Structural Insights into the Interaction Between Adenovirus C5 Hexon and Human Lactoferrin. Dhillon A, Persson BD, Volkov AN, Sulzen H, Kadek A, Pompach P, Kereiche S, Lepsik M, Danskog K, Uetrecht C, Arnberg N, Zoll S. (2024) J Virol, e0157623. doi: 10.1128/jvi.01576-23
- RNA to Rule Them All: Critical Steps in Lassa Virus Ribonucleoparticle Assembly and Recruitment. Sänger L, Williams HM, Yu D, Vogel D, Kosinski J, Rosenthal M#, Uetrecht C# (2023) J Am Chem Soc, https://doi.org/10.1021/jacs.3c07325 #corresponding authors
- Commissioning the digital mass-filter/ion-trap module for the MS SPIDOC prototype. Simke F, Fischer P, Damjanović T, Kádek A, Kierspel T, Lorenzen K, Uetrecht C, Schweikhard L (2023) J Instrum, 18 P10021 https://doi.org/10.1088/1748-0221/18/10/P10021.
- Fucose binding cancels out mechanical differences between norovirus strains. Feng Y, Pogan R, Thiede L, Müller-Guhl J, Uetrecht C, Roos WH (2023) Viruses, 15, 1482 doi: 10.3390/v15071482
- A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells. Soh TK, Pfefferle S, Wurr S, von Possel R, Oestereich L, Rieger T, Uetrecht C, Rosenthal M, Bosse JB (2023) PLoS One 18(5):e0274065. doi: 10.1371/journal.pone.0274065
- Coherent Diffractive Imaging of proteins and viral capsids: Simulating MS SPIDOC. Kierspel T#, Kadek A, Barran P, Bellina B, Bijedic A, Bordmerkel M, Commandeur J, Caleman C, Damjanovic T, Dawod I, De Santis E, Lekkas A, Lopez Morillo L, Lorenzen K, Mandl T, Marklund EG, Papanastasiou D, Ramakers LAI, Schweikhard L, Simke F, Sinelnikova A, Smyrnakis A, Timneanu N, Uetrecht C# for the MS SPIDOC consortium (2023) Anal Bioanal Chem, https://doi.org/10.1007/s00216-023-04658-y, Forefront #corresponding authors
- Stability and Conformational Memory of Electrosprayed and Rehydrated Bacteriophage MS2 Virus Coat Proteins. Brodmerkel MN, De Santis E, Caleman C, Uetrecht C, Marklund EG. (2022) Curr Res Struct Biol. 4:338-348. doi: 10.1016/j.crstbi.2022.10.001
- In-solution structure and oligomerization of human histone deacetylase 6 - an integrative approach. Shukla S, Komarek J, Novakova Z, Nedvedova J, Ustinova K, Vankova P, Kadek A, Uetrecht C, Mertens H, Barinka C. (2023) FEBS J, 290(3):821-836. doi: 10.1111/febs.16616.
- NMR experiments provide insights into ligand-binding to the SARS-CoV-2 spike protein receptor-binding domain. Creutznacher R, Maass T, Veselkova B, Ssebyatika G, Krey T, Empting M, Tautz N, Frank M, Koelbel K, Uetrecht C, Peters T. (2022) J Am Chem Soc, 144(29):13060-13065. doi: 10.1021/jacs.2c05603.
- Distinct dissociation rates of murine and human norovirus P domain dimers suggest a role of dimer stability in virus-host interactions. Creutznacher R, Maass T, Dülfer J, Feldmann C, Hartmann V, Lane MS, Knickmann J, Westermann LT, Thiede L, Smith TJ, Uetrecht C, Mallagaray A, Waudby CA, Taube S, Peters T. (2022) Commun Biol, 5(1):563 https://doi.org/10.1038/s42003-022-03497-4.
- Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain Dimers. Dirscherl C, Loechte S, Hein Z, Kopicki JD, Harders AR, Linden N, Karner A, Preiner J, Weghuber J, Garcia-Alai M, Uetrecht C, Zacharias M, Piehler J, Lanzerstorfer P, Springer S. (2022) J Cell Sci, jcs.259498.
- Opening opportunities for Kd determination and screening of MHC peptide complexes. Kopicki JD, Saikia A, Niebling S, Günther C, Anjanappa R, Garcia-Alai MM, Springer S#, Uetrecht C#. (2022) Commun Biol, 5:488, doi: 10.1038/s42003-022-03366-0, #corresponding authors
- Assignment of Ala, Ile, LeuproS, Met, and ValproS methyl groups of the protruding domain of murine norovirus capsid protein VP1 using methyl-methyl NOEs, site directed mutagenesis, and pseudocontact shifts. Maass T, Westermann LT, Creutznacher R, Mallagaray A, Dülfer J, Uetrecht C, Peters T. (2022) Biomol NMR Assign, DOI: 10.1007/s12104-022-10066-7
- Caldendrin/myosinV regulate spine apparatus localization via ER-stabilization in dendritic spines. Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M. (2022) EMBO J, 41:e106523, doi: 10.15252/embj.2020106523
- Protein secondary structure affects glycan clustering in native mass spectrometry. Yan H, Lockhauserbäumer J, Szekeres GP, Mallagaray A, Creutznacher R, Taube S, Peters T, Pagel K, Uetrecht C. (2021) Life, 11:554.
- Glycan-induced protein dynamics in human norovirus P dimers depend on virus strain and deamidation status. Dülfer J, Yan H, Brodmerkel MN, Creutznacher R, Mallagaray A, Peters T, Caleman C, Marklund EG, Uetrecht C. (2021) Molecules, 26:2125.
- X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease. Günther S, Reinke PYA, Fernández-García Y, Lieske J, Lane TJ, Ginn HM, Koua FHM, Ehrt C, Ewert W, Oberthuer D, Yefanov O, Meier S, Lorenzen K, Krichel B, Kopicki JD, Gelisio L, Brehm W, Dunkel I, Seychell B, Gieseler H, Norton-Baker B, Escudero-Pérez B, Domaracky M, Saouane S, Tolstikova A, White TA, Hänle A, Groessler M, Fleckenstein H, Trost F, Galchenkova M, Gevorkov Y, Li C, Awel S, Peck A, Barthelmess M, Schlünzen F, Xavier PL, Werner N, Andaleeb H, Ullah N, Falke S, Srinivasan V, Alves Franca B, Schwinzer M, Brognaro H, Rogers C, Melo D, Zaitsev-Doyle JJ, Knoska J, Peña Murillo GE, Rahmani Mashhour A, Hennicke V, Fischer P, Hakanpää J, Meyer J, Gribbon P, Ellinger B, Kuzikov M, Wolf M, Beccari AR, Bourenkov G, von Stetten D, Pompidor G, Bento I, Panneerselvam S, Karpics I, Schneider TR, Garcia Alai MM, Niebling S, Günther C, Schmidt C, Schubert R, Han H, Boger J, Monteiro DCF, Zhang L, Sun X, Pletzer-Zelgert J, Wollenhaupt J, Feiler CG, Weiss MS, Schulz EC, Mehrabi P, Karničar K, Usenik A, Loboda J, Tidow H, Chari A, Hilgenfeld R, Uetrecht C, Cox R, Zaliani A, Beck T, Rarey M, Günther S, Turk D, Hinrichs W, Chapman HN, Pearson AR, Betzel C, Meents A. (2021) Science, 372:642-646
- Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes. Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C. (2021) Sci Adv, 7:eabf1004.
- N-terminal VP1 truncations favor T=1 norovirus-like particles. Pogan R, Weiss VU, Bond K, Dülfer J, Krisp C, Lyktey N, Mueller-Guhl J, Zoratto S, Allmaier G, Jarrold MF, Munoz-Fontela C, Schlüter H, Uetrecht C. (2020) Vaccines, 9:8
- The XBI BioLab for life science experiments at the European XFEL. Han H, Round E, Schubert R, Gül Y, Makroczyova J, Meza D, Heuser P, Äpfelbacher M, Barak I, Betzel C, Fromme P, Kursula I, Nissen P, Tereschenko E, Schulz J, Uetrecht C, Ulicny J, Wilmanns M, Hajdu J, Lamzin VS, Lorenzen K. (2021) J Appl Cryst, 54:7-21.
- The crystal structure of mycobacterial Epoxide Hydrolase A. Schulz EC, Henderson SR, Illarionov B, Crosskey T, Southall SM, Krichel B, Uetrecht C, Fischer M, Wilmanns M. (2020) Sci Rep, 10:16539.
- Megahertz single-particle imaging at the European XFEL. Sobolev E, Zolotarev S, Giewekemeyer K, Bielecki J, Okamoto K, Reddy HKN, Andreasson J, Ayyer K, Barak I, Bari S, Barty A, Bean R, Bobkov S, Chapman HN, Chojnowski G, Daurer BJ, Dörner K, Ekeberg T, Flückiger L, Galzitskaya O, Gelisio L, Hauf S, Hogue BG, Horke DA, Hosseinizadeh A, Ilyin V, Jung C, Kim C, Kim Y, Kirian RA, Kirkwood H, Küpper J, Kulyk O, Letrun R, Loh D, Messerschmidt M, Mühlig K, Ourmazd A, Raab N, Rode AV, Rose M, Round A, Sato T, Schubert R, Schwander P, Sellberg JA, Sikorski M, Silenzi A, Song C, Spence JCH, Stern S, Sztuk-Dambietz J, Teslyuk A, Timneanu N, Trebbin M, Uetrecht C, Weinhausen B, Williams GJ, Xavier PL, Xu C, Vartanyants I, Lamzin V, Mancuso A, Maia FRNC. (2020) Commun Phys, 3:97.
- Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion. Horstmann JA, Lunelli M, Cazzola H, Heidemann J, Kühne C, Steffen P, Szefs S, Rossi C, Lokareddy RK, Wang C, Hughes KT, Uetrecht C, Schlüter H, Grassl GA, Stradal TEB, Rossez Y, Kolbe M, Erhardt M. (2020) Nat Commun, 11:2013.
- Processing of the SARS-CoV pp1a/ab nsp7-10 region. Krichel B, Falke S, Hilgenfeld R, Redecke L, Uetrecht C. (2020) Biochem J, 477(5):1009-1019. Cover article.
- Structures of peptide-free and partially loaded MHC class I molecules reveal mechanisms of peptide selection. Anjanappa R, Garcia-Alai M, Kopicki J-D, Lockhauserbäumer J, Aboelmagd M, Hinrichs J, Nemtanu IM, Uetrecht C, Zacharias M, Springer S, Meijers R. (2020) Nat Commun, 11:1314.
- Further insights from structural mass spectrometry into endocytosis adaptor protein assemblies. Heidemann J, Kölbel K, Konijnenberg A, Van Dyck J, Garcia-Alai M, Meijers R, Sobott F, Uetrecht C. (2020) Int J Mass Spectrom, 447: 116240. Cover article, IJMS Best Student Paper Award 2019.
- Structural analysis of ligand-bound states of the Salmonella type III secretion system ATPase InvC. Bernal I, Römermann J, Flacht L, Lunelli M, Uetrecht C, Kolbe M. (2019) Protein Sci, 28:1888-1901.
- Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. Bernal I§, Börnicke J§, Heidemann J§, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A#, Uetrecht C#, Kolbe M#. (2019) J Mol Biol, 431:3787-3803. §equal contribution, #corresponding authors
- Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA). Weiss VU§, Pogan R§, Zoratto S, Bond K, Boulanger P, Jarrold MF, Lyktey N, Pahl D, Puffler N, Schelhaas M, Selivanovitch E, Uetrecht C, Allmaier G. (2019) Anal Bioanal Chem, 411:5951-5962. §equal contribution
- Synthesis and characterization of α-carboxynitrobenzyl photocaged L-aspartates for applications in time-resolved structural biology. Zaitsev-Doyle JJ, Puchert A, Pfeifer Y, Yan H, Yorke BA, Müller-Werkmeister HM, Uetrecht C, Rehbein J, Huse N, Pearson AR, Sans M. (2019) RSC Advances, 9, 8695-8699.
- Native mass spectrometry provides sufficient ion flux for XFEL single particle imaging. Uetrecht C#, Lorenzen K, Kitel M, Heidemann J, Spencer JHR, Schlüter H, Schulz J. (2019) J Synchrotron Radiat, 26:653-659. #corresponding author
- Crystal structure of a domain-swapped photoactivatable sfGFP variant provides evidence for GFP folding pathway. Kesgin-Schaefer S, Heidemann J, Puchert A, Koelbel K, Yorke B, Huse N, Pearson AR, Uetrecht C, Tidow H. (2019) FEBS J, 286:2329-2340.
- A post-translational modification of human Norovirus capsid protein attenuates glycan binding. Mallagaray A, Creutznacher R, Dülfer J, Mayer P, Grimm L, Orduña J, Trabjerg E, Stehle T, Rand K, Blaum B, Uetrecht C, Peters T. (2019) Nat Commun, 10:1320.
- Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H. (2018) Commun Biol, 1:206.
- Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses. Holm T, Kopicki JD, Busch C, Olschewski S, Rosenthal M, Uetrecht C, Günther S, Reindl S. (2018) J Biol Chem, 293(51):19686-19698.
- Fucose-Functionalized Precision Glycomacromolecules Targeting Human Norovirus Capsid Protein. Bücher KS, Yan H, Creutznacher R, Ruoff K, Mallagaray A, Grafmüller A, Dirks JS, Kilic T, Weickert S, Rubailo A, Drescher M, Schmidt S, Hansman G, Peters T, Uetrecht C#, Hartmann L#. (2018) Biomacromolecules, 19(9):3714-3724. #corresponding authors
- Norovirus-like VP1 particles exhibit isolate dependent stability profiles. Pogan R, Schneider C, Reimer R, Hansman G, Uetrecht C. (2018) J Phys Condens Matter, 30:064006.
- Epsin and Sla2 form assemblies through phospholipid interfaces. Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C#, Meijers R#. (2018) Nat Comm, 9:328. #corresponding authors
- Ultra-high mass multimer analysis of protein-1a capping domains by a silicon nanomembrane detector. Shin HC, Deterra D, Park J, Kim H, Nishikiori M, Uetrecht C, Ahlquist PG, Arbulu M, Blick RH. (2017) J Proteomics 175:5-11.
- Human norovirus GII.4(MI001) P dimer binds fucosylated and sialylated carbohydrates. Wegener H, Mallagaray Á, Schöne T, Peters T, Lockhauserbäumer J, Yan H, Uetrecht C, Hansman GS, Taube S. (2017) Glycobiology, 27:1027-1037.
- A data set from flash X-ray imaging of carboxysomes. Hantke MF, Hasse D, Ekeberg T, John K, Svenda M, Loh D, Martin AV, Timneanu N, Larsson DS, van der Schot G, Carlsson GH, Ingelman M, Andreasson J, Westphal D, Iwan B, Uetrecht C, Bielecki J, Liang M, Stellato F, DePonte DP, Bari S, Hartmann R, Kimmel N, Kirian RA, Seibert MM, Mühlig K, Schorb S, Ferguson K, Bostedt C, Carron S, Bozek JD, Rolles D, Rudenko A, Foucar L, Epp SW, Chapman HN, Barty A, Andersson I, Hajdu J, Maia FR. (2016) Sci Data, 3:160061.
- Structure and target interaction of a G-quadruplex RNA-aptamer. Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U. (2016) RNA Biol, 13:973-987.
- Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. (2016) Biomacromolecules, 17:2522-9.
- Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R. (2016) J Biol Chem, 291:4882-93.
- Attachment of norovirus to histo blood group antigens: a cooperative multistep process. Mallagaray A, Lockhauserbäumer J, Hansman G, Uetrecht C, Peters T. (2015) Angew Chem Int Ed Engl, 54:12014-9.
- A hydrolase-based reporter system to uncover the protein splicing performance of an archaeal intein. von der Heyde A, Lockhauserbäumer J, Uetrecht C, Elleuche S. (2015) Appl Microbiol Biotechnol, 99:7613-24.
- An organized co-assembly of clathrin adaptors is essential for endocytosis. Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M. (2015) Dev Cell, 33:150-62.
- Game-theory-based search engine to automate the mass assignment in complex native electrospray mass spectra. Tseng YH, Uetrecht C, Yang SC, Barendregt A, Heck AJ, Peng WP. (2013) Anal Chem, 85:11275-83.
- Probing the biophysical interplay between a viral genome and its capsid. Snijder J, Uetrecht C, Rose RJ, Sanchez-Eugenia R, Marti GA, Agirre J, Guérin DM, Wuite GJ, Heck AJ, Roos WH. (2013) Nat Chem, 5:502-9.
- Single amino acid exchange in bacteriophage HK620 tailspike protein results in thousand-fold increase of its oligosaccharide affinity. Broeker NK, Gohlke U, Müller JJ, Uetrecht C, Heinemann U, Seckler R, Barbirz S. (2013) Glycobiology, 23:59-68.
- Structural studies of the O-antigen polysaccharide from Escherichia coli TD2158 having O18 serogroup specificity and aspects of its interaction with the tailspike endoglycosidase of the infecting bacteriophage HK620. Zaccheus MV, Broeker NK, Lundborg M, Uetrecht C, Barbirz S, Widmalm G. (2012) Carbohydr Res, 357:118-25.
- Prestress strengthens the shell of Norwalk virus nanoparticles. Baclayon M, Shoemaker GK, Uetrecht C, Crawford SE, Estes MK, Prasad BV, Heck AJ, Wuite GJ, Roos WH. (2011) Nano Lett, 11:4865-9.
- Encapsulation of phthalocyanine supramolecular stacks into virus-like particles. Brasch M, de la Escosura A, Ma Y, Uetrecht C, Heck AJ, Torres T, Cornelissen JJ. (2011) J Am Chem Soc, 133:6878-81.
- Interpreting the charge state assignment in electrospray mass spectra of bioparticles. Tseng YH, Uetrecht C, Heck AJ, Peng WP. (2011) Anal Chem, 83:1960-8.
- Interrogating viral capsid assembly with ion mobility-mass spectrometry. Uetrecht C, Barbu IM, Shoemaker GK, van Duijn E, Heck AJ. (2011) Nat Chem, 3:126-32. Erratum in 2014: Nat Chem, 6:745.
- N-terminal domain of human Hsp90 triggers binding to the cochaperone p23. Karagöz GE, Duarte AM, Ippel H, Uetrecht C, Sinnige T, van Rosmalen M, Hausmann J, Heck AJ, Boelens R, Rüdiger SG. (2011) Proc Natl Acad Sci USA, 108:580-5.
- Squeezing protein shells: how continuum elastic models, molecular dynamics simulations, and experiments coalesce at the nanoscale. Roos WH, Gibbons MM, Arkhipov A, Uetrecht C, Watts NR, Wingfield PT, Steven AC, Heck AJ, Schulten K, Klug WS, Wuite GJ. (2010) Biophys J, 99:1175-81.
- Subunit exchange rates in Hepatitis B virus capsids are geometry- and temperature-dependent. Uetrecht C, Watts NR, Stahl SJ, Wingfield PT, Steven AC, Heck AJ. (2010) Phys Chem Chem Phys, 12:13368-71. Cover article.
- Norwalk virus assembly and stability monitored by mass spectrometry. Shoemaker GK, van Duijn E, Crawford SE, Uetrecht C, Baclayon M, Roos WH, Wuite GJ, Estes MK, Prasad BV, Heck AJ. (2010) Mol Cell Proteomics, 9:1742-51.
- A docking model based on mass spectrometric and biochemical data describes phage packaging motor incorporation. Fu CY, Uetrecht C, Kang S, Morais MC, Heck AJ, Walter MR, Prevelige PE Jr. (2010) Mol Cell Proteomics, 9:1764-73.
- Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related. Barbirz S, Müller JJ, Uetrecht C, Clark AJ, Heinemann U, Seckler R. (2008) Mol Microbiol, 69:303-316. Cover article.
- Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly. Lorenzen K, Olia AS, Uetrecht C, Cingolani G, Heck AJ. (2008) J Mol Biol, 379:385-396.
- High-resolution mass spectrometry of viral assemblies: molecular composition and stability of dimorphic hepatitis B virus capsids. Uetrecht C, Versluis C, Watts NR, Roos WH, Wuite GJ, Wingfield PT, Steven AC, Heck AJ. (2008) Proc Natl Acad Sci USA, 105:9216-9220.
- Stability and shape of hepatitis B virus capsids in vacuo. Uetrecht C, Versluis C, Watts NR, Wingfield PT, Steven AC, Heck AJ. (2008) Angew Chem Int Ed Engl, 47:6247-6251. Inner cover.
Peer-review review articles
- Norovirus—A Viral Capsid in Perpetual Flux. Thiede L, Pogan R, Uetrecht C. (2023) In: Comas-Garcia, M., Rosales-Mendoza, S. (eds) Physical Virology in Springer Series Biophysics. https://doi.org/10.1007/978-3-031-36815-8_8
- Norovirus-glycan interactions - How strong are they really? Peters T, Creutznacher R, Maass T, Mallagaray A, Ogrissek P, Taube S, Thiede L, Uetrecht C. (2021) Biochem Soc Trans, 50:347-359. doi: 10.1042/BST20210526.
- In a flash of light: X-ray free electron lasers meet native mass spectrometry. Kádek A, Lorenzen K, Uetrecht C for the MS SPIDOC consortium. (2021) Drug Discov Today Technol, 39:89-99.
- Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology. Simanjuntak Y, Schamoni-Kast K, Grün A, Uetrecht C#, Scaturro P#. (2021) Viruses. 13:668 #corresponding authors
- Structural mass spectrometry goes viral. Dülfer J, Kadek A, Kopicki JD, Krichel B, Uetrecht C. (2019) Adv Virus Res, 105:189-238. Cover article.
- Norovirus assembly and stability. Pogan R, Dülfer J, Uetrecht C. (2018) Curr Opin Virol, 31:59-65.
- Modern biomolecular mass spectrometry and its role in studying virus structure, dynamics, and assembly. Uetrecht C, Heck AJ. (2011) Angew Chem Int Ed Engl, 50:8248-62.
- Ion mobility mass spectrometry of proteins and protein assemblies. Uetrecht C, Rose RJ, van Duijn E, Lorenzen K, Heck AJ. (2010) Chem Soc Rev, 39:1633-55.
Manuscripts without peer-review
- Automated high-throughput selection of DNA aptamers using a common optical next-generation sequencer. Drees A, Ahlers C, Kehrer T, Ehmke N, Grün AFR, Uetrecht C,Ignatova Z, Schumacher U, Fischer M. (2024) bioRxiv doi: https://doi.org/10.1101/2024.06.24.600375
- Subtleties in Clathrin Heavy Chain Binding Boxes provide selectivity among Adaptor Proteins of Budding Yeast. Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Köbel K, Uetrecht C, Thuenauer R, García-Alai MM. (2024) bioRxiv doi: https://doi.org/10.1101/2024.05.30.596561
- Overview of the 2023 Physical Virology Gordon Research Conference—Viruses at Multiple Levels of Complexity. Hagan MF, Zandi R, Uetrecht C (2024) Viruses 16(6), 895; https://doi.org/10.3390/v16060895
- Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility. Feng Y§, van Bodegraven D§, Kádek A§, Munguira ILB, Soria-Martinez L, Nentwich S, Kavan D, Uetrecht C#, Schelhaas M#, Roos WH#. (2024) bioRxiv doi: https://doi.org/10.1101/2024.02.01.577804#corresponding authors, §equal contribution
- The kinetics of SARS-CoV-2 nsp7-11 polyprotein processing and impact on complexation with nsp16. Schamoni-Kast K, Krichel B, Damjanovic T, Kierspel T, Toker S, Uetrecht C. (2024) bioRxiv https://doi.org/10.1101/2024.01.06.574466
- Norovirus Superstar (in English or German). Mischitz V. Editors: Cross A, Wissel S, Uetrecht C, Lorenzen K. Comic book of for the EU project MS SPIDOC. Doi: 10.22003/XFEL.EU-COMIC-2021-001-EN / 10.22003/XFEL.EU-COMIC-2021-001-DE
- SARS-CoV-2 induced CYP19A1 expression in the lung correlates with increased aromatization of testosterone-to-estradiol in male golden hamsters. Stanelle-Bertram S, Schaumburg B, Mounogou Kouassi N, Beck S, Zickler M, Beythien G, Becker K, Bai T, Jania H, Müller Z, Pinho dos Reis V, Krump-Buzumkic V, Kadek A, Uetrecht C, Schroeder M, Jarczak D, Nierhaus A, Kluge S, Scaturro P, Baumgärtner W, Klingel K, Gabriel G. researchsquare, doi: 10.21203/rs.3.rs-107474/v1
- The COVID-19 MS Coalition—accelerating diagnostics, prognostics, and treatment. Struwe W, Emmott E, Bailey M, Sharon M, Sinz A, Corrales FJ, Thalassinos K, Braybrook J, Mills C, Barran P behalf of the COVID-19 MS Coalition. (2020) Lancet, P1761-1762, DOI:https://doi.org/10.1016/S0140-6736(20)31211-3
- Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease. Günther S, Reinke PYA, Oberthuer D, Yefanov O, Ginn H, Meier S, Lane TJ, Lorenzen K, Gelisio L, Brehm W, Dunkel I, Domaracky M, Saouane S, Lieske J, Ehrt C, Koua F, Tolstikova A, White TA, Groessler M, Fleckenstein H, Trost F, Galchenkova M, Gevorkov Y, Li C, Awel S, Peck A, Xavier PL, Barthelmess M, Schlünzen F, Werner N, Andaleeb H, Ullah N, Falke S, Alves Franca B, Schwinzer M, Brognaro H, Seychell B, Gieseler H, Melo D, Zaitsev-Doyle JJ, Norton-Baker B, Knoska J, Esperanza G, Rahmani Mashhour A, Guicking F, Hennicke V, Fischer P, Rogers C, Monteiro DCF, Hakanpää J, Meyer J, Noei H, Gribbon P, Ellinger B, Kuzikov M, Wolf M, Zhang L, Sun X, Pletzer-Zelgert J, Wollenhaupt J, Feiler C, Weiss M, Schulz EC, Mehrabi P, Schmidt C, Schubert R, Han H, Krichel B, Fernández-García Y, Escudero-Pérez B, Günther S, Turk D, Uetrecht C, Beck T, Tidow H, Chari A, Zaliani A, Rarey M, Cox R, Hilgenfeld R, Chapman HN, Pearson AR, Betzel C, Meents A. (2020) biorxiv, doi: doi.org/10.1101/2020.05.02.043554
- Native Massenspektrometrie für die Proteinstrukturanalytik. Heidemann J, Krichel B, Uetrecht C. (2018) BIOspektrum, 24:164-167.
- Sample refreshment schemes for high repetition rate FEL experiments. Schulz J, Bari S, Buck J, Uetrecht C.(2013) Proceedings Volume 8778, Advances in X-ray Free-Electron Lasers II: Instrumentation; 87780T.
Prof. Dr. Charlotte Uetrecht
Gebäude 61,
Raum 314
Email: | charlotte.uetrecht(at)uni-luebeck.de |
Phone: | +49 451 3101-3300 |
Professor for Chemistry (W3)
Universität zu Lübeck (UzL), Lübeck
Deutsches Elektronen-Synchrotron DESY, and Centre for Structural Systems Biology (CSSB), Hamburg, Germany
ORCID-ID: 0000-0002-1991-7922